polyclonal chicken igy anti-mouse bdnf  (Promega)

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Animal treatment protocol. Mice were sensitised to OVA adsorbed to Al(OH)3 or vehicle alone by intraperitoneal injections on days 1, 14 and 21. Prior to analysis, animals received two consecutive local aerosol challenges of 1% OVA (w v−1) diluted in PBS or PBS alone and delivered by 20-min aerosolisation on days 26 and 27. Intranasal application of polyclonal chicken IgY (isotype antibody) or anti-mouse BDNF was performed 3 h before each airway allergen challenge. In addition, animals received the antibodies i.p. on day 25. The response to acute allergen exposure was measured on day 35 in the body plethysmograph. All animals were analysed 24 h after the last challenge. Abbreviations: intraperitoneally (i.p.), intranasally (i.n.), aerosol challenge (aerosol).

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Aerosol

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Overview of experiments and animal numbers

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Aerosol

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of anti-BDNF treatment on allergen-induced airway obstruction by HBP. Measurement of expiratory flow in response to OVA (intranasal application during continuous measurement of airflow in the HBP). (a) Integration of EF50 values between 15 and 25 min after OVA application. (b) Time course of n=11 animals in each group. Data from three separate experiments are shown. Grey bar: possible artefacts from OVA application. The error bars represent standard error of the mean (s.e.m.). Student́s t-test: ***P<0.001.

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques:

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of BDNF and anti-BDNF treatment on inflammation. (a, b) Cytokine levels in BALF. IL-4, IL-5 and IFN-γ were measured in BALF from nonsensitised or OVA-sensitised mice 24 h after aerosol challenge by ELISA. The BAL recovery was 1.4±0.2 ml in all groups. (c, d) Cell differentiation in BALF after OVA aerosol challenge on day 28. Lymphocytes, macrophages, eosinophils and neutrophils were differentiated according to morphological criteria in BALF from nonsensitised or OVA-sensitised mice 24 h after the last aerosol challenge. (e) Cell differentiation in BALF 24 h after nasal OVA challenge on day 36. Student's t-test did not reveal any statistically significant difference (P<0.05) between groups. In all, 12 mice were analysed in each group, in two separate experiments. The error bars represent standard deviation (s.d.).

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Aerosol, Enzyme-linked Immunosorbent Assay, Cell Differentiation, Standard Deviation

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of BDNF on airway hyper-responsiveness in response to EFS. Airway hyper-responsiveness was measured in response to EFS. The frequency that caused 50% of maximal airway smooth muscle constriction was defined as ES50. Airway contractility was expressed as mean±s.d. Statistics were performed using t-test with Welch's correction. P<0.05 was regarded as a statistically significant difference. (a) Nonsensitised BALB/c mice were treated intranasally either with recombinant human BDNF (5 μg ml−1, 50 μl) or BSA (0.1%, 50 μl) as a control on two consecutive days. AHR was measured 24 h after the last treatment. In all, 12 animals were examined in each group in two separate experiments. (a) OVA-sensitised Balb/c mice were treated intranasally either with anti-BDNF (500 μg ml−1, 50 μl) or istotype IgY (500 μg ml−1, 50 μl) as a control on two consecutive days, 3 h before OVA aerosol challenge. In addition, the animals received 100 μl of the antibody solution i.p. 24 h before the first OVA challenge (day 25). AHR was measured 24 h after the last treatment. A total of 12 animals were examined in each group in two separate experiments.

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: Recombinant, Control, Aerosol

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Effect of anti-BDNF treatment on airway hyper-responsiveness in response to methacholine and capsaicin by HBP. (a) Dose–response curve of expiratory flow (EF50) in response to inhaled methacholine. (b) Dose–response curve of expiratory flow (EF50) in response to inhaled capsaicin. (c) Dose–response curve of sensory irritation (TB) in response to inhaled methacholine. (d) Dose–response curve of sensory irritation (TB) in response to inhaled capsaicin. The error bars represent standard error of the mean (s.e.m.), n=8–10 animals in each group in three separate experiments. Student's t-test: *P<0.05. (a) Nonsensitised against OVA-sensitised+isotype, (d) OVA-sensitised+isotype against OVA-sensitised+anti-BDNF.

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques:

Journal:

Article Title: Brain-derived neurotrophic factor (BDNF) contributes to neuronal dysfunction in a model of allergic airway inflammation

doi: 10.1038/sj.bjp.0705638

Figure Lengend Snippet: Tachykinin containing sensory neurons from ovalbumin-sensitised mice

Article Snippet: Polyclonal chicken IgY anti-mouse BDNF (2 × 25 μ l) (500 μ g ml −1 ) or isotype antibody (500 μ g ml −1 ) (Promega, Madison, U.S.A.) solved in sterile PBS was instilled intranasally 3 h before each airway allergen challenge.

Techniques: